Please use this identifier to cite or link to this item: http://repository.psau.edu.sa:80/jspui/handle/123456789/5018
Title: Fisetin and telmisartan each alone or in low-dose combination alleviate OVA-induced food allergy in mice
Authors: Mohamed Fatehy Hassan Balaha
Reem Elkholy
Noha El-Anwar
Samah Kandeel
Sabiha Hedya
Mohamed-Nabih Abd-El Rahman
Keywords: Fisetin and telmisartan each alone or in low-dose combination alleviate OVA-induced food allergy in mice
Issue Date: Dec-2018
Publisher: Pharmacological Reports
Abstract: Background: Food allergy (FA) is a worldwide health problem, affecting nearly 10% of all populations, with no prophylactic options or regulatory treatment available until now. Fisetin, a biologically active flavonoid, and telmisartan, the highly selective competitive AT1 receptor antagonist, recently exhibited potent anti-inflammatory and immunomodulatory activities. In the present study, we have evaluated the possible anti-inflammatory and immunomodulatory activities of fisetin and telmisartan each alone or in low-dose combination in a mouse model of FA. Methods: For induction of FA, eight-week-old BALB/c mice, sensitized by two ip injection of 50 mg ovalbumin (OVA) and 1 mg alum at day 0 and 7. Then, each mouse challenged with 10 mg OVA at days 14, 16, 18, and 21. On the 28th day, the fifth challenge carried out by oral administration of 50 mg OVA. Either fisetin (1 or 3 mg/kg/d), telmisartan (1 or 3 mg/kg/d) or a combination of fisetin 1 mg/kg/d and telmisartan 1 mg/kg/d received orally from the 13th day till 28th day. In challenge days, the treatments received one-hour before the challenge. Results: Our data showed that fisetin and telmisartan each alone or in low-dose combination attenuated the anaphylactic manifestation, decreased blood eosinophilic count, serum OVA-specific IgE, and IL-4 levels, the intestinal total and degranulated mast cells count, and CD4+ immunohistochemical expression. Furthermore, they enhanced the serum IFN-g level and abrogated the intestinal histopathological changes induced by OVA in mice. Conclusion: Either fisetin, telmisartan or their low-dose combination could be promising in the management of FA.
Description: Background: Food allergy (FA) is a worldwide health problem, affecting nearly 10% of all populations, with no prophylactic options or regulatory treatment available until now. Fisetin, a biologically active flavonoid, and telmisartan, the highly selective competitive AT1 receptor antagonist, recently exhibited potent anti-inflammatory and immunomodulatory activities. In the present study, we have evaluated the possible anti-inflammatory and immunomodulatory activities of fisetin and telmisartan each alone or in low-dose combination in a mouse model of FA. Methods: For induction of FA, eight-week-old BALB/c mice, sensitized by two ip injection of 50 mg ovalbumin (OVA) and 1 mg alum at day 0 and 7. Then, each mouse challenged with 10 mg OVA at days 14, 16, 18, and 21. On the 28th day, the fifth challenge carried out by oral administration of 50 mg OVA. Either fisetin (1 or 3 mg/kg/d), telmisartan (1 or 3 mg/kg/d) or a combination of fisetin 1 mg/kg/d and telmisartan 1 mg/kg/d received orally from the 13th day till 28th day. In challenge days, the treatments received one-hour before the challenge. Results: Our data showed that fisetin and telmisartan each alone or in low-dose combination attenuated the anaphylactic manifestation, decreased blood eosinophilic count, serum OVA-specific IgE, and IL-4 levels, the intestinal total and degranulated mast cells count, and CD4+ immunohistochemical expression. Furthermore, they enhanced the serum IFN-g level and abrogated the intestinal histopathological changes induced by OVA in mice. Conclusion: Either fisetin, telmisartan or their low-dose combination could be promising in the management of FA.
URI: http://repository.psau.edu.sa:80/jspui/handle/123456789/5018
ISSN: 1734-1140
Appears in Collections:College of Pharmacy AL-Kharj

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